Glyco-PAINT

Sugar molecules tagged with a fluorescent marker (in red) are binding reversibly to the mannose receptors anchored on the cell membrane, which allows tracking of their movements and provides information on binding affinity and receptor mobility in living cells. Riera, R., Hogervorst, T.P., Doelman, W. et al. Single-molecule imaging of glycan–lectin interactions on cells with Glyco-PAINT. Nat Chem Biol 17, 1281–1288 (2021).